| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1413-2 | ||||
Resumo:Mastitis is one of the main diseases that affect dairy herds and can cause numerous losses in the milk production chain, mainly due to decreased production, treatment costs, disposal of contaminated milk, and compromised milk quality. This disease is characterized by an inflammatory process of the mammary gland and can be caused by bacteria, fungi, and viruses. Effective control of mastitis, whether clinical or subclinical, is essential to ensure the health of the dairy herd and the quality of milk production. Therefore, the aim of this study was to diagnose mastitis and identify the bacteria causing the disease on dairy farms in the Northern Region of Espírito Santo. For this purpose, ten milk-producing farms were selected, and a total of 100 cows at different lactation stages were analyzed. The presence of clinical mastitis was assessed by background dark mug test, examining for the presence of clots, blood, or pus in the milk. Subclinical mastitis was evaluated using the rapid test California Mastitis Test (CMT) following the manufacturer's recommendations. Ten milk samples from cows diagnosed with mastitis were selected for analysis of the microbiological profile. Isolation and identification of major bacterial species were carried out by culturing different media and using primary and secondary biochemical tests. The results showed that 34.5% of the evaluated animals had mastitis. Subclinical mastitis was diagnosed in 22% of the animals, while 12.5% had clinical mastitis. The predominance of subclinical mastitis found in this study represents a problem for farmers because, besides not showing clinical signs, this disease can be contagious and can easily spread among the herd. The results of the microbiological profile of the raw milk samples showed that Staphylococcus spp. (33.33%), Pseudomonas sp. (33.33%), Streptococcus sp. (16.67%), and Escherichia (16.67%) were the predominant genera in the samples. These bacteria are pathogenic and described in the literature as the main cause of mastitis. Its mostly originate from the environment, highlighting the importance of hygiene measures and proper animal management to prevent mastitis. Identifying the microbial profile of mastitis-causing bacteria allows the rational use of antibiotics in disease treatment, prevents antibiotic residues in milk, selection of resistant bacteria and reduces losses due to inadequate treatments. Effective control of mastitis is fundamental to the sustainability and profitability of dairy herds. Preventive measures, such as good management practices, hygiene, and constant monitoring, are essential to prevent and control mastitis. Palavras-chave: Bovine Mastitis, Pathogenic Bacteria, Milk Quality, Clinical Mastitis, Subclinical Mastitis. Agęncia de fomento:Centro Universitario do Espírito Santo UNESC | |||||